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| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases |
| Topic 2: Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Physical, chemical, and microscopic analysis - Cerebrospinal, serous, and synovial fluids - Urine formation and composition |
| Topic 3: Hematology | 20-25% | - Hemostasis and coagulation testing - White blood cell morphology and diseases - Routine and automated hematology procedures - Red blood cell physiology and disorders |
| Topic 4: Blood Banking (Immunohematology) | 15-20% | - Transfusion reactions and adverse events - Compatibility testing - Blood group systems and antigens - Antibody detection and identification - Blood components and storage |
| Topic 5: Microbiology | 15-20% | - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Specimen collection and culture techniques - Mycology, parasitology, and virology |
| Topic 6: Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Laboratory safety and regulatory compliance - Quality control and quality assurance |
| Topic 7: Immunology and Serology | 5-10% | - Immune system principles and components - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders - Infectious disease serology |
1. Only non-self antigens can be immunogenic. Self antigens are normally recognized by the immune system as part of the host, so an immune response does not normally occur. Non-self antigens are immunogenic since they have the potential to cause an immune response.
For a substance to be immunogenic it must be:
A) A linear molecule
B) A lipid
C) Recognized as non-self
D) A haptene
E) Less than 5,000 molecular weight
2. Escherichia coli and Klebsiella pneumoniae are two good quality control choices for the indole test. E. coli is indole positive, while K. pneumoniae is indole negative. A positive reaction is noted when there is a red layer at the top of the tube after the addition of Kovács reagent, while the negative result is a lack of color change in the top of the tube after the addition of Kovács reagent.
Which one of the following combinations of organisms would be appropriate positive and negative controls to verify the specific test functions listed?
A) Beta hemolysis - Escherichia coli and Streptococcus pyogenes
B) Hydrogen sulfide production - proteus mirabilis and Salmonella typhi
C) Catalase - Staphylococcus aureus and Staphylococcus epidermidis
D) Indole - Escherichia coli and Klebsiella pneumonia
E) Carbohydrate fermentation - Alcaligenes faecalis and Acinetobacter baumannii
3. Failure to deliver glucose drawn in a serum separator tube (SST) to the laboratory within the recommended time will cause:
Question options:
A) a falsely increased glucose value
B) both A and C
C) glycolysis
D) a falsely decreased glucose value
4. Which of the following tubes produces a serum sample?
Question options:
A) pink
B) tiger (red-gray)
C) blue
D) green
5. To make a 1 : 10 dilution, 270 µL of diluent should be added to 30 µL of sample, .
A spinal fluid that is slightly hazy is briefly examined microscopically. The technologist performing the count decides to make a 1:10 dilution using 30 µL of sample. What volume of diluent should be used?
A) 70 µL
B) 300 µL
C) 270 µL
Solutions:
| Question # 1 Answer: C | Question # 2 Answer: D | Question # 3 Answer: B | Question # 4 Answer: B | Question # 5 Answer: C |
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