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American Society of Microbiology ABMM Sample Questions:

1. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) Multilocus sequence typing (MLST)
B) Whole-genome sequencing (WGS) SNP analysis
C) PCR Ribotyping
D) Pulsed-field gel electrophoresis (PFGE)

2. A microbiology laboratory receives a CSF sample from a patient with suspected fungal meningitis. India ink staining reveals encapsulated yeast cells. Culture on Sabouraud dextrose agar grows creamy white colonies.
The MOST likely organism is:

A) Mucor species
B) Candida albicans
C) Cryptococcus neoformans
D) Aspergillus flavus

3. A patient with a history of travel to a tropical region presents with cyclical fevers and chills.Peripheral blood smear reveals the presence of merozoites and schizonts within erythrocytes. The absence of crescent-shaped gametocytes makes Plasmodium falciparum less likely. The MOST probable species is:

A) Plasmodium knowlesi
B) Plasmodium malariae
C) Plasmodium ovale
D) Plasmodium vivax

4. A patient presents with perianal itching, particularly at night. Microscopic examination of cellophane tape preparation from the perianal area reveals oval-shaped eggs with one flattened side. The MOST likely causative organism is:

A) Strongyloides stercoralis
B) Enterobius vermicularis
C) Trichuris trichiura
D) Ascaris lumbricoides

5. A patient with a history of chronic lung disease develops a severe pneumonia. Bronchoalveolar lavage fluid reveals the presence of numerous cysts containing multiple nuclei, which stain positively with silver stains but poorly with Gram stain. The patient's CD4+ T-cell count is within the normal range. The MOST likely causative organism and the preferred treatment are:

A) Histoplasma capsulatum; amphotericin B followed by itraconazole.
B) Paragonimus westermani; praziquantel.
C) Blastomyces dermatitidis; itraconazole.
D) Pneumocystis jirovecii; trimethoprim-sulfamethoxazole.

Solutions: